ETS & Oxidative Phosphorylation

Redox Energy Calculation:

• Calculate the efficiency of energy capture in the oxidation of NADH by the electron transport system. KEY

 

Consider the relative reduction pattern of the ETS in the presence of NADH (highly reduced) oxygen (highly oxidized) and low [ADP].

What would a plot of reduction vs. ETS components (e.g. NADH, FADH₂, CoQH₂, cyt C, etc.) look like? KEY

Calculations of P/O ratios

Calculate the P/O ratio (ATPs produced for each oxygen atom consumed) for the aerobic catabolism of glucose in mammalian fast twitch muscle. Show all work in a table such as we have used in class. KEY

Do this calculation again for glucose in insect flight muscle. KEY

• Why should this difference exist? (That is, why should evolution favor this lower value of energy for insect flicht muscle?)

Fatty Acid Oxidation

Calculate the P/O ratio for the aerobic catabolism of stearate (C=18). Show all work in a table such as we have used in class. KEY

 

From the Final Exam Study Guide:

Electron Transport System

• Know localization.
• Know order of major components in ETS starting with
  • NADH
  • Succinate
  • Glycerol-P
  • Fatty acyl CoA
  • How many ATP's are normally produced for each of these starting materials.
• What kinds of evidence do we have for ordering of components.
• Where is ATP production associated?
• What are:
  • cytochromes
  • flavoproteins
  • Non Heme Iron proteins
  • CoQ?
• Know what functional groups or cofactors are used by various conponents.
• Be able to explain and/or diagram the model we discussed for the coupling of electron transport and ox. phos. (chemiosmotic).
• Be able to calculate P/O ratios
  • for any compounds oxidized via the ETS.
  • for the aerobic catabolism of any compound given, or for which you know, its pathway of metabolism.
• Be able to trace the electron shuttle systems
  • malate-aspartate
  • glycerol phosphate
• How is ATP/ADP transported across the inner mitochodrial membrane.