 Laboratory |
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| Chem 432 | ||
| Spring 2002 | Tentative | Office: SA470b |
| Office Hours: M, W 2; other times by appointment |
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Phone: x 5719 Home: 822-1116 |
| e-mail: rap1@axe |
Required Text: Ninfa & Ballou. Fundamentals Laboratory Approaches for Biochemistry and Biotechnology, Fitzgerald Science Press (1998)
Attendance: Attendance in the laboratory is required. You can not pass this course (lecture and lab) if you miss 3 or more labs. laboratory Since the introductory lecture at the beginning of each laboratory includes safety hazards and protocols as well as experimental procedures, you must be present for the introductory lecture. Many reagents have short lifetimes, are expensive, and must be made specifically for each laboratory experiment. Thus experiments may not be made up.
Supplies: Approved Eye-Protection (see safety regulations) and a bound Laboratory Notebook are required. Nitrile goves, Sharpie™ markers or equivalent, and a lab coat are recommended. I will provide used floppy disks for free upon request.
Lab Reports: I am still determining speciifcs for lab reports - watch this space!
| Week | Date |
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Reading** |
| 1 | 1/22 | Laboratory Safety; Check-in. Library - research isolation method for mitochondria! | |
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Introduction to Molecular Modeling - Small Molecules | Handouts: Mol Mod 1, Mol Mod II |
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Continue Molecular Modeling - Small Molecules | Handouts: Mol Mod III |
| 3 |
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Bring in Mitochondrial Isolation Proceedures for discussion Thursday!! (You should also have preliminary lists of materials, apparatus, chemicals etc. so we can get this started ASAP) | |
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| 5 |
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Lab Lecture: Characterization of Macromolecules: Size and Shape - Sedimentation Methods. | |
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Lab Lecture: Characterization of Macromolecules: Size and Shape - Chromatography | |
| 10 |
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Lab Lecture: Characterization of Macromolecules: Size and Shape - Chromatography, cont. | |
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Isolation of DNA - |
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| 11 |
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Lab Lecture: Characterization of Macromolecules: Size and Shape - Chromatography, cont. | |
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Charecterization of DNA |
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Lab Lecture: Introduction to Spectroscopy | |
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Isolation of Plant Pigments |
| Molecular Modeling | Report form provided. | |
| Isolation and Characterization of Mitochondria | Complete article as per Biochemistry. (Go to library and check out Biochemistry instructions to authors for details- also available on-line via library periodicals list.) | Due Friday, April 5 |
| Isolation and Charecterization of DNA | Complete Report as per Science (Go to library and check out Science instructions to authors for details, and look at some typical reports in the journal as examples.) | Due Monday, April 22 |
| Isolation of Plant Pigments | Complete Report as per Science (Go to library and check out Science instructions to authors for details, and look at some typical reports in the journal as examples.) | Due Friday, May 17 |
| Laboratory |
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Last modified 7 May 2002
