Presentation Year

2024

Depreciated Participant

Jenny Cappuccio Chemistry Staffjc3534@humboldt.edu<br>David Morales Chemistry Undergraduate Student dam29@humboldt.edu<br>Valeria Aviles Chemistry Undergraduate Student va96@humboldt.edu<br>Frank Cappuccio Chemistry Faculty fc412@humboldt.edu

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Rhodopsins are light-sensitive proteins within the G-protein coupled receptor (GPCR) family that convert photons into intracellular chemical signals that perform downstream biological functions. Anabaena sensory rhodopsin (ASR) with a 6x-His tag was isolated and purified using Ni-NTA affinity chromatography after evaluating clones of induced E. coli transformed with a pET15b plasmid. The most viable clones discovered from this study were BU3E and Box A. SDS-PAGE gel electrophoresis confirmed the protein size of 20.65 kDa. These results indicate that these 2 strains can be utilized for further studies on ASR function in nanodiscs.

Permission to Publish Work

Yes

Primary Contact: First Name

Jacquelyn

Primary Contact: Last Name

Amadeo-Ranch

Primary Contact: Email

jwa41@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

9253146013

Node ID

1567

Page Classification