Breadcrumb
Characterization of a Novel Endocellulase Enzyme for Biofuel Optimization
Presentation Year
2024
College or Department
College of Natural Resources & Sciences
Short Description of your Research or Creative Project (700 characters or less)
Currently food crops are used to produce bioethanol production, while plant waste cellulose could be used. However, cellulase enzymes are a limiting factor. We sought to characterize a novel cellulase identified by metagenomic analysis of bovine rumen by the JGI and Hess et al. We performed protein expression, genomic analysis, and characterization by SDS-PAGE, CMC and DNS activity assays, and electron microscopy of cellulose degradation. Our data indicates the cellulase is an endoglucanase with an activity of 6.27 cm2/ug, or 6x higher than commercial cellulase enzymes. These results have implications for creating efficient biofuels from agricultural waste products versus the current methods
Does your project align with any of the following?
sustainability related (explores at least two dimensions of sustainability: environment, economy, society)
Permission to Publish Work
Yes
Primary Contact: First Name
Amanda
Primary Contact: Last Name
Ratcliff
Primary Contact: Email
ajr172@humboldt.edu
Primary Contact: I am a
Undergraduate Student
Primary Contact: Phone Number
925-266-7074