Analysis of a G-Protein Coupled Receptor, CB2

Presentation Year

2023

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

The CB2 G-protein coupled receptors (GPCR) is found in the brainstem & hippocampus and is devoid of psychotropic effects but is less studied than the CB1 receptor. CB2 is inducible in CNS microglia following inflammation or injury, indicating a role in pain response. Here we sought to analyze CB2 using ChimeraX structures and overcome GPCR protein insolubility in extraction. The pET28a-CNR2, plasmid created and transformed into E.coli pLysS, was confirmed by restriction digest. Purification of CB2 micelles was achieved by affinity chromatography with detergent (43 kD). We aim to utilize nanodiscs to stabilize CB2, allowing studies of the molecular underpinnings informing treatment options.

Permission to Publish Work

Yes

Primary Contact: First Name

David

Primary Contact: Last Name

Lopez

Primary Contact: Email

Dml100@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

3237917202