ASR Membrane Protein and ApoA1 Detection in Nanodisc via Western Blot Analysis

Presentation Year

2022

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Anabaena sensory rhodopsin (ASR) is a prokaryotic photoactive transmembrane G protein coupled receptor extracted from the cyanobacterium Nostoc. The potential for ASR and its transducer, ASRT, to transcribe/translate prokaryotic genes into products by light signals is an extremely attractive possibility for future membrane research. Nanodiscs are a useful tool for studying membrane proteins because they serve to create a stable amphipathic environment. In the present study, 6x-His ASR has been extracted, isolated, purified, and inserted into a MSP nanodisc with ApoA1 and DMPC. We examine ApoA1 insertion into the nanodisc via SDS-PAGE and chemiluminescent western blot analysis.

Permission to Publish Work

Yes