Breadcrumb
Deciphering the Crosstalk within Human Coronary Atherosclerotic Plaque
Presentation Year
2022
College or Department
College of Natural Resources & Sciences
Short Description of your Research or Creative Project (700 characters or less)
Our project investigates the cross-talk between cells contained within the human coronary atherosclerotic plaque using single-cell technologies.
We mapped the transcriptome of the plaque using 10x transcriptomics and validated our findings with immunohistochemistry and in vitro assays.
We found a predominance of memory T cells suggesting T cells were recruited by antigen engagement with their cognate peptide presented by myeloid cells.
Using in vitro assays, we also found activation of pro-inflammatory and pro-fibrotic pathways in smooth muscle cells after exposure to T cell cytokines.
Our analysis suggests that T cells communicate with myeloid and smooth muscle cells within the plaque
We mapped the transcriptome of the plaque using 10x transcriptomics and validated our findings with immunohistochemistry and in vitro assays.
We found a predominance of memory T cells suggesting T cells were recruited by antigen engagement with their cognate peptide presented by myeloid cells.
Using in vitro assays, we also found activation of pro-inflammatory and pro-fibrotic pathways in smooth muscle cells after exposure to T cell cytokines.
Our analysis suggests that T cells communicate with myeloid and smooth muscle cells within the plaque
Permission to Publish Work
Yes