Developing a three-dimensional culture system to improve the yield of placental mesenchymal stem cell derived extracellular vesicles

Presentation Year

2022

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

In this study, we developed a novel approach to produce placental mesenchymal stem cell derived extracellular vesicles (PMSC-EVs) by utilizing a three-dimensional (3D) cell culture system to potentially use in regenerative medicine applications. CELLine bioreactors are a 3D culture system that is comprised of a polystyrene scaffold, to support a high-density culture, encased in a semipermeable membrane to concentrate all proteins produced by the cells within. When conditioned medium is collected from the compartment PMSC-EVs were produced over an eight-week continuous culture. This method potentially could be used as an alternative to conventional culture methods to improve PMSC-EV yield.

Permission to Publish Work

Yes