Development and Stable Integration of Tetracycline Inducible Tools for Genome Editing and Genome Regulation in Cultured Human Embryonic Kidney Cells

Presentation Year

2019

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

At Humboldt State University we aim to create a robust CRISPR/Cas9 toolkit which enables one to overexpress, repress, inhibit transcription and perform double strand DNA cuts allowing one to undertake research on genes associated with cancer, autophagy and neurological diseases. These tools will enable future students, private sector, and academia to investigate a wide range of biological questions. Currently in the CRISPR/Cas9 system there are inherent problems ranging from indel mutations, poor gene locus targeting, low efficiency, and in vivo applications.We built upon the knowledge in Dr. Steele’s lab and chose to perform Hi-Fi assembly to overcome technological limitations that lowered.

Permission to Publish Work

No