Electroporation of E. coli and Agar Gel Electrophoresis of FB5⍺

Presentation Year

2022

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Chitin is the second-most abundant polysaccharide after cellulose, and a rich source of nitrogen and reduced carbons which is currently underutilized. Chitinases have the potential to put this sustainable resource to use. In this experiment a plasmid purported to contain the ChiA endochitinase gene was extracted from FB5-alpha E. coli and transformed into BL21(DE3) E. coli. The plasmid was analyzed by agar gel electrophoresis to determine that the gene was not present in the plasmid. To confirm the lack of the gene, protein isolation will be attempted, and samples will be analyzed by SDS-PAGE

Permission to Publish Work

Yes