Breadcrumb
Evaluation of a Novel Cellulase to Optimize Biofuel Production
Presentation Year
2023
College or Department
College of Natural Resources & Sciences
Short Description of your Research or Creative Project (700 characters or less)
Cellulose waste is utilized for biofuels, however cellulase enzymes are a limiting factor. We sought to evaluate a novel cellulase identified in cow rumen metagenomic analysis provided by the Joint Genome Institute. We performed DNA analysis, SDS PAGE, and a cellulase activity assay to theoretically calculate and compare to experimental molecular weight; and determine cellulase activity. The theoretical molar mass (64.9 kD) strongly correlates to the experimental molar mass (60.7 kDa). Purified cellulase has an activity, 6.27 cm2/ug, 6x higher versus commercial cellulase enzyme. These results have positive implications for the creation of biofuels from agricultural waste products.
Does your project align with any of the following?
sustainability (explores interrelationships between environment society AND economy)
Permission to Publish Work
Yes
Primary Contact: First Name
Amanda
Primary Contact: Last Name
Ratcliff
Primary Contact: Email
ajr172@humboldt.edu
Primary Contact: I am a
Undergraduate Student
Primary Contact: Phone Number
925-266-7074