Breadcrumb
Expression, Purification & Functional Comparison of MSP1D1 vs. Apo-AI in ASR Nanodisc Assembly
Presentation Year
2026
College or Department
College of Natural Resources & Sciences
Short Description of your Research or Creative Project (700 characters or less)
This project investigates whether MSP1D1 can replace Apo-A1 as the scaffold protein in ASR(anabaena sensory rhodopsin) nanodisc assembly. MSP1D1 is first expressed in E.coli, then purified using His-tag/Ni-NTA affinity chromatography, and is then incorporated into nanodiscs with purified ASR and phospholipid. The resulting MSP1D1-based nanodiscs are then compared with Apo-A1 based nanodiscs using SDS-PAGE gels, Native-PAGE gels, and dynamic light scattering(DLS). The goal is to determine whether MSP1D1 can form stable, uniform nanodiscs and function as an effective alternative scaffold for membrane protein purification and analysis.
Permission to Publish Work
Yes
Presentation File Upload
Primary Contact: First Name
Andrew
Primary Contact: Last Name
Jenkins Cruz
Primary Contact: Email
agj20@humboldt.edu
Primary Contact: I am a
Undergraduate Student
Primary Contact: Phone Number
4157304624
Indicate File Dimensions
4'x3'