Functional impact of alternative splicing on the transcriptomic landscape and fate of multipotent skeletal stem cells and osteosarcoma

Presentation Year

2022

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Greater than 90% of all protein-coding genes in the human genome undergo post-translational alternative splicing (AS), giving rise to many unique isoforms from a single gene. Our work with human skeletal stem cell (hSSC) has demonstrated the need to examine AS as it relates to cancer, development, aging, skeletal regeneration and skewing of hSSCs towards non-skeletogenic lineage fates. Here we explored the relationship between AS and function of hSSCs. Using RNA microarray technology we discovered RNA-binding proteins involved in hSSC function; inhibition of these proteins prevents differentiation and formation of osteosarcoma. Our current work reveals other essential roles of AS in hSSCs.

Permission to Publish Work

Yes