Isolation & Nanodisc Assembly of Submitochondrial Particles

Presentation Year

2022

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

The mitochondrion is a subcellular location for varied metabolic processes and pathways relevant to undergraduate biochemistry courses at Cal Poly Humboldt. At the core of such pathways are integral membrane proteins (IMPs) whose structure and enzymatic activity can be examined to better understand not only their overall function, but also the role of specific subunits. One such enzyme is cytochrome c oxidase (complex IV). Nanodiscs are invaluable tools for examining IMPs. In this experiment, we isolated mitochondria and submitochondrial particles for incorporation into nanodiscs. Future work will include purification and selection for nanodiscs containing only cytochrome c oxidase.

Permission to Publish Work

Yes