Lipidomic Analysis of Anabaena 7120 using 2D Aqueous Polymer Fractionation and LCMS Analysis

Presentation Year

2026

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Anabaena PCC 7120 is a species of cyanobacteria containing a light sensing membrane protein, Anabaena Sensory Rhodopsin (ASR) which is under investigation in our nanobiotechnology lab. Lipids greatly affect the activity of membrane proteins, but the native lipids of Anabaena are largely unknown. Here we sought to determine the composition of these membrane lipids to enable future studies into ASR. In this study we seek to identify the specific lipidome of the thylakoid and plasma membrane of Anabaena using a 2D aqueous polymer to density gradient extraction, followed by analysis with liquid chromatography – mass spectrometry.

Permission to Publish Work

Yes

Presentation File Upload

chemistry-guillot-miles-203435-4x3.pdf (15.7 KB)

Primary Contact: First Name

Miles

Primary Contact: Last Name

Guillot

Primary Contact: Email

mcg139@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

2063888978

Indicate File Dimensions

4'x3'