Breadcrumb
Progress towards Cloning of the ASRT Gene from Anabaena into the pET-28a Expression Vector
Presentation Year
2026
College or Department
College of Natural Resources & Sciences
Short Description of your Research or Creative Project (700 characters or less)
This project focuses on the molecular cloning of the ASRT gene from Anabaena into the pET-28a expression vector for propagation in Escherichia coli. Genomic DNA was extracted from an Anabaena culture and used as a template for PCR amplification of the ASRT coding sequence using specifically designed primers. The amplified gene will be inserted into pET-28a using restriction enzyme cloning, followed by transformation into E. coli. This approach enables stable maintenance of the gene and provides a foundation for future expression and analysis.
Permission to Publish Work
Yes
Presentation File Upload
Primary Contact: First Name
Catherine
Primary Contact: Last Name
Maloney
Primary Contact: Email
cgm65@humboldt.edu
Primary Contact: I am a
Undergraduate Student
Primary Contact: Phone Number
5305655497
Indicate File Dimensions
4'x3'