Presentation Year

2022

Depreciated Participant

Nicole L Womack College of Natural Resources & Sciences Undergraduate Student, Hannah Cornwell Undergraduate Student, David Lopez Undergraduate Student, Dylan Morris Undergraduate Student

College or Department

Short Description of your Research or Creative Project (700 characters or less)

The G-protein coupled receptor (GPCR), CB2, is part of a widespread neuromodulatory system found in all animals and is suspected to play a role in both CNS and immune system functions, yet it is a relatively understudied. We demonstrated a successful method for the expression and purification of the GPCR CB2 protein from BL21(DE3) pLysS competent cells. This method involves the culturing of an E. coli BL21(DE3) pLysS MBP-CB2-6xHis cell line, induction of CB2 receptor expression via IPTG, and the harvest and isolation of expressed CB2 proteins via Ni-NTA Immobilized Metal Affinity Chromatography. This method was proven to be successful based on SDS-page and spectrophotometric analyses.

Permission to Publish Work

Yes

Node ID

1116

Page Classification