Presentation Year

2025

Depreciated Participant

Mason Menna Chemistry Undergraduate Student mgm140@humboldt.edu<br>Raul Pacheco Chemistry Undergraduate Student rp205@humboldt.edu<br>Aidan Childs Chemistry Undergraduate Student amc316@humboldt.edu<br>Jenny Cappuccio Chemistry Faculty jc3534@humboldt.edu

College or Department

Short Description of your Research or Creative Project (700 characters or less)

Anabaena sensory rhodopsin (ASR) is a light-sensitive transmembrane protein found in the cyanobacterium Anabaena Nostoc. Membrane proteins have proven difficult to study due to their insolubility and tendency to form aggregates in aqueous environments. Nanodiscs are small protein structures that assist in the study of insoluble proteins. In this experiment, self-assembled ASR-bound nanodiscs were purified by Ni-NTA Immobilized Metal Affinity Chromatography (IMAC). Native gel electrophoresis, SDS-PAGE, western blotting, and absorption spectroscopy were used to detect Anabaena sensory rhodopsin and the scaffolding protein, ApoAI, in the prepared nanodisc sample.

Permission to Publish Work

Yes

Primary Contact: First Name

Celeste

Primary Contact: Last Name

Diepenbrock

Primary Contact: Email

cad131@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

(916)809-6755

Node ID

1759

Page Classification