Characterization of Anabaena Sensory Rhodopsin Bound Nanodiscs via Western Blotting

Presentation Year

2025

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Anabaena sensory rhodopsin (ASR) is a light-sensitive transmembrane protein found in the cyanobacterium Anabaena Nostoc. Membrane proteins have proven difficult to study due to their insolubility and tendency to form aggregates in aqueous environments. Nanodiscs are small protein structures that assist in the study of insoluble proteins. In this experiment, self-assembled ASR-bound nanodiscs were purified by Ni-NTA Immobilized Metal Affinity Chromatography (IMAC). Native gel electrophoresis, SDS-PAGE, western blotting, and absorption spectroscopy were used to detect Anabaena sensory rhodopsin and the scaffolding protein, ApoAI, in the prepared nanodisc sample.

Permission to Publish Work

Yes

Primary Contact: First Name

Celeste

Primary Contact: Last Name

Diepenbrock

Primary Contact: Email

cad131@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

(916)809-6755