Breadcrumb
Engineering Ionically Crosslinked Alginate-Gelatin Granular Hydrogels for 3D Cell Culture
Presentation Year
2026
Kariun
Steven
Undergraduate Student
Chemistry
Jacob
Costa
Undergraduate Student
Chemistry
Naomi
Smith
Undergraduate Student
Chemistry
College or Department
College of Natural Resources & Sciences
Short Description of your Research or Creative Project (700 characters or less)
3D cell culture substrates often require complex, costly chemical modifications, limiting accessibility. To address this, we developed a novel granular hydrogel platform using purely ionically crosslinked alginate and gelatin. By exploiting gelatin's inherent RGD motifs for cell integrin binding, we engineered microbeads of varying alginate-gelatin ratios to assess their influence on HEK 293T cell viability, adhesion, and morphology. These 3D granular matrices were compared to 2D hydrogels of identical compositions to evaluate substrate surface effects on cells. This simple, chemical-free gelation method significantly enhances the accessibility of complex 3D tissue engineering models.
Permission to Publish Work
Yes
Presentation File Upload
chemistry-barela-marcus-203721-3x2.pdf (8.76 KB)
Primary Contact: First Name
Marcus
Primary Contact: Last Name
Barela
Primary Contact: Email
mab361@humboldt.edu
Primary Contact: I am a
Undergraduate Student
Primary Contact: Phone Number
5304170552
Indicate File Dimensions
3'x2'