Light and Dark Assay Comparison of Selected Strains of Anabaena Sensory Rhodopsin

Presentation Year

2025

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Anabaena Sensory Rhodopsin (ASR) is a retinal containing membrane protein from the cyanobacteria, Anabaena (Nostoc) PCC 7120. The ASR protein undergoes a conformational change upon absorption of orange light, which causes the all-trans-retinal to be converted to 13-cis retinal and the release of the associated transducer protein (ASRT). In order to facilitate future nanolipoprotein studies we examined the efficacy of the production of ASR in several selected recombinant strains of E. Coli through a light and dark spetrophotometric analysis of the purified ASR protein.

Permission to Publish Work

Yes

Primary Contact: First Name

Miles

Primary Contact: Last Name

Guillot

Primary Contact: Email

mcg139@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

2063888978