Prevention of Escherichia coli Biofilm Formation Using SLpA Protein Extracted From Lactobacillus helveticus

Presentation Year

2026

College or Department

College of Natural Resources & Sciences

Short Description of your Research or Creative Project (700 characters or less)

Dental health in pets is often neglected, leading to gingivitis and periodontitis. Probiotics are thought to inhibit oral pathogenic bacteria through surface layer proteins, preventing biofilm formation and occupying binding sites. We explored Lactobacillus helveticus and its protein SLpA in preventing biofilm formation in mCherry expressing Escherichia coli. SLpA was extracted using lithium chloride and centrifugation. E. coli was mixed with L. helvetius and SLpA. Fluorescence values were collected with a sodium alginate and calcium chloride gel in each well to allow bacterial adherence and compared to a blank. Results show L. helveticus and SLpA inhibit biofilm formation of E. coli.

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Permission to Publish Work

Yes

Presentation File Upload

chemistry-renner-scarlet-203780-4x3.pdf (1.83 MB)

Primary Contact: First Name

Scarlet

Primary Contact: Last Name

Renner

Primary Contact: Email

smr210@humboldt.edu

Primary Contact: I am a

Undergraduate Student

Primary Contact: Phone Number

7074970200

Indicate File Dimensions

4'x3'